Fabian Roth, Markus Numberger, Andreas Draguhn Patch clamp technique Springer-Verlag, Heidelberg. 2023 https://doi.org/10.1007/978-3-662-66053-9 ISBN: 978-3-662-66052-2
Today, the patch clamp technique is one of the most important neurophysiological working methods. Its application in basic biomedical research has yielded significant insights into the function and properties of ion channels, and it has also become indispensable in applied pharmacological research. In a clear and practical manner, the authors provide the essential information to introduce and successfully apply the method in the laboratory. The book explains the theoretical basics of the measurement, the detailed setup of a measuring station and the practical implementation of typical measurement configurations. Concrete tips and tricks help with "trouble shooting" and are an indispensable aid not only for new users.
Back text
Today, the patch clamp technique is one of the most important neurophysiological working methods. Its application in basic biomedical research has yielded significant insights into the function and properties of ion channels, and it has also become indispensable in applied pharmacological research.
Published by Springer-Nature, this book is the new edition of the popular, but long out-of-print, German-language introduction to the patch clamp technique. Much has changed and developed since the first edition. For example, almost all of the illustrations are new, and this also applies to most of the chapters. Some things, such as the special applications in Chap. 6 or Chap. 7 on the documentation and processing of data, are new additions.
The original approach of creating a practical guide and a teaching aid that really makes the work easier for both teachers and students has remained. In a clear and practical manner, the authors provide the essential information to introduce and successfully apply the method in the laboratory. The book explains the theoretical basics of measurement, the detailed setup of a measurement setup, and the practical implementation of typical measurement configurations. Concrete tips and tricks help with "trouble shooting" and are an indispensable aid not only for new users. In addition, the enormous potential of the technique is highlighted, not only for the investigation of ion channels and cell signals, but also the most important further developments and variants of the patch clamp technique are addressed.
The book is intended for advanced students of biology, biochemistry, medicine, pharmacy, and related fields, as well as scientists who want to better understand and/or acquire the technology.
The authors
Fabian Roth is a neurobiologist and conducts research and teaches at the Institute for Basic Medical Research (IMB) at the University of Oslo.
Markus Numberger is a biologist and works as a freelance science writer in the fields of medicine, pharmacy, and biology.
Andreas Draguhn is a physician and university lecturer at Heidelberg University Hospital of the Ruprecht-Karls-Universität Heidelberg.